Your browser doesn't support javascript.
loading
Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming.
Liu, Xiaodong; Nefzger, Christian M; Rossello, Fernando J; Chen, Joseph; Knaupp, Anja S; Firas, Jaber; Ford, Ethan; Pflueger, Jahnvi; Paynter, Jacob M; Chy, Hun S; O'Brien, Carmel M; Huang, Cheng; Mishra, Ketan; Hodgson-Garms, Margeaux; Jansz, Natasha; Williams, Sarah M; Blewitt, Marnie E; Nilsson, Susan K; Schittenhelm, Ralf B; Laslett, Andrew L; Lister, Ryan; Polo, Jose M.
Afiliação
  • Liu X; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Nefzger CM; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
  • Rossello FJ; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Chen J; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Knaupp AS; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
  • Firas J; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Ford E; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Pflueger J; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
  • Paynter JM; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Chy HS; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • O'Brien CM; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
  • Huang C; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Mishra K; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Hodgson-Garms M; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
  • Jansz N; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Williams SM; Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Blewitt ME; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
  • Nilsson SK; Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
  • Schittenhelm RB; ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, Perth, Western Australia, Australia.
  • Laslett AL; The Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
  • Lister R; ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, Perth, Western Australia, Australia.
  • Polo JM; The Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
Nat Methods ; 14(11): 1055-1062, 2017 Nov.
Article em En | MEDLINE | ID: mdl-28945704
ABSTRACT
Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Reprogramação Celular Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Reprogramação Celular Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2017 Tipo de documento: Article País de afiliação: Austrália