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Ectopic expression of UGT84A2 delayed flowering by indole-3-butyric acid-mediated transcriptional repression of ARF6 and ARF8 genes in Arabidopsis.
Zhang, Gui-Zhi; Jin, Shang-Hui; Li, Pan; Jiang, Xiao-Yi; Li, Yan-Jie; Hou, Bing-Kai.
Afiliação
  • Zhang GZ; The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, 250100, China.
  • Jin SH; School of Pharmacy, Linyi University, Linyi, 276000, China.
  • Li P; The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, 250100, China.
  • Jiang XY; School of Life Science, Qingdao Agricultural University, Qingdao, 266109, China.
  • Li YJ; The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, 250100, China.
  • Hou BK; The Key Lab of Plant Cell Engineering and Germplasm Innovation, Ministry of Education of China, School of Life Science, Shandong University, Jinan, 250100, China.
Plant Cell Rep ; 36(12): 1995-2006, 2017 Dec.
Article em En | MEDLINE | ID: mdl-29027578
KEY MESSAGE: Ectopic expression of auxin glycosyltransferase UGT84A2 in Arabidopsis can delay flowering through increased indole-3-butyric acid and suppressed transcription of ARF6, ARF8 and flowering-related genes FT, SOC1, AP1 and LFY. Auxins are critical regulators for plant growth and developmental processes. Auxin homeostasis is thus an important issue for plant biology. Here, we identified an indole-3-butyric acid (IBA)-specific glycosyltransferase, UGT84A2, and characterized its role in Arabidopsis flowering development. UGT84A2 could catalyze the glycosylation of IBA, but not indole-3-acetic acid (IAA). UGT84A2 transcription expression was clearly induced by IBA. When ectopically expressing in Arabidopsis, UGT84A2 caused obvious delay in flowering. Correspondingly, the increase of IBA level, the down-regulation of AUXIN RESPONSE FACTOR 6 (ARF6) and ARF8, and the down-regulation of flowering-related genes such as FLOWERING LOCUS T (FT), SUPPRESSOR OF OVEREXPRESSION OF CO1(SOC1), APETALA1 (AP1), and LEAFY(LFY) were observed in transgenic plants. When exogenously applying IBA to wild-type plants, the late flowering phenotype, the down-regulation of ARF6, ARF8 and flowering-related genes recurred. We examined the arf6arf8 double mutants and found that the expression of flowering-related genes was also substantially decreased in these mutants. Together, our results suggest that glycosyltransferase UGT84A2 may be involved in flowering regulation through indole-3-butyric acid-mediated transcriptional repression of ARF6, ARF8 and downstream flowering pathway genes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Arabidopsis / Proteínas de Arabidopsis / Proteínas de Ligação a DNA / Glucosiltransferases / Indóis Tipo de estudo: Prognostic_studies Idioma: En Revista: Plant Cell Rep Assunto da revista: BOTANICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Arabidopsis / Proteínas de Arabidopsis / Proteínas de Ligação a DNA / Glucosiltransferases / Indóis Tipo de estudo: Prognostic_studies Idioma: En Revista: Plant Cell Rep Assunto da revista: BOTANICA Ano de publicação: 2017 Tipo de documento: Article País de afiliação: China