Toward Genome-Based Metabolic Engineering in Bacteria.
Adv Appl Microbiol
; 101: 49-82, 2017.
Article
em En
| MEDLINE
| ID: mdl-29050667
ABSTRACT
Prokaryotes modified stably on the genome are of great importance for production of fine and commodity chemicals. Traditional methods for genome engineering have long suffered from imprecision and low efficiencies, making construction of suitable high-producer strains laborious. Here, we review the recent advances in discovery and refinement of molecular precision engineering tools for genome-based metabolic engineering in bacteria for chemical production, with focus on the λ-Red recombineering and the clustered regularly interspaced short palindromic repeats/Cas9 nuclease systems. In conjunction, they enable the integration of in vitro-synthesized DNA segments into specified locations on the chromosome and allow for enrichment of rare mutants by elimination of unmodified wild-type cells. Combination with concurrently developing improvements in important accessory technologies such as DNA synthesis, high-throughput screening methods, regulatory element design, and metabolic pathway optimization tools has resulted in novel efficient microbial producer strains and given access to new metabolic products. These new tools have made and will likely continue to make a big impact on the bioengineering strategies that transform the chemical industry.
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Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Genoma Bacteriano
/
Engenharia Metabólica
Idioma:
En
Revista:
Adv Appl Microbiol
Ano de publicação:
2017
Tipo de documento:
Article
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