Development of a microextraction by packed sorbent with gas chromatography-mass spectrometry method for quantification of nitroexplosives in aqueous and fluidic biological samples.
J Sep Sci
; 41(3): 639-647, 2018 Feb.
Article
em En
| MEDLINE
| ID: mdl-29086477
ABSTRACT
A new method for quantification of 12 nitroaromatic compounds including 2,4,6-trinitrotoluene, its metabolites and 2,4,6-trinitrophenyl-N-methylnitramine with microextraction by packed sorbent followed by gas chromatography and mass spectrometric detection in environmental and biological samples is developed. The microextraction device employs 4 mg of C18 silica sorbent inserted into a microvolume syringe for sample preparation. Several parameters capable of influencing the microextraction procedure, namely, number of extraction cycles, washing solvent, volume of washing solvent, elution solvent, volume of eluting solvent and pH of matrix, were optimized. The developed method produced satisfactory results with excellent values of coefficient of determination (R2 > 0.9804) within the established calibration range. The extraction yields were satisfactory for all analytes (> 89.32%) for aqueous samples and (> 87.45%) for fluidic biological samples. The limits of detection values lie in the range 14-828 pg/mL.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trinitrotolueno
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Substâncias Explosivas
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Microextração em Fase Sólida
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Limite de Detecção
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Cromatografia Gasosa-Espectrometria de Massas
Limite:
Humans
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Índia