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Aberrant expression of thyroid transcription factor-1 in schwannomas.
Wang, Dai-Zhong; Liu, Ping; Yao, Li; Hao, Ying-Hua; Zhu, Rui-Juan; Zhang, Tao; Tang, Xian-Bin.
Afiliação
  • Wang DZ; Department of Pathology, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000.
  • Liu P; Department of Pathology, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000.
  • Yao L; Department of Pathology, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000.
  • Hao YH; Department of Pathology, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000.
  • Zhu RJ; Department of Pathology, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000.
  • Zhang T; Department of Neurosurgery, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000.
  • Tang XB; Department of Pathology, Taihe Hospital, Hubei University of Medicine, Hubei, China 442000. Electronic address: taiheblk@126.com.
Hum Pathol ; 71: 84-90, 2018 01.
Article em En | MEDLINE | ID: mdl-29104109
Aberrant expression of thyroid transcription factor-1 (TTF-1) has been observed in tumors arising in locations other than thyroid gland, lung and ventral forebrain. However, TTF-1 expression in schwannomas has not yet been studied. Meanwhile, a few inconsistent changes in protein expression have been identified between schwannomas and other peripheral nerve sheath tumors. We evaluated TTF-1 expression in 161 schwannomas and 43 other peripheral nervous system lesions, including ganglioneuromas (n = 8), malignant peripheral nerve sheath tumors (MPNSTs) (n = 11), neurofibromas (n = 24), and traumatic neuromas (n = 9), using immunohistochemistry and verified it using quantitative real-time reverse-transcription polymerase chain reaction (qPCR) to explore TTF-1 expression in peripheral nervous system lesions. Formalin-fixed paraffin-embedded (FFPE) tissues were obtained for both analyses. In this study, we observed nuclear TTF-1 staining in 109 (67.7%) schwannomas, including 102 of 131 (77.9%) conventional, 1 of 20 (5.0%) cellular and 6 of 10 (60.0%) plexiform schwannomas. Nuclear staining was not observed in normal peripheral nerves and non-schwannoma lesions. qPCR verified the aberrant expression and revealed a correlation between TTF-1 protein and mRNA levels (r = 0.633, P = .003). In conclusion, the data from our study show that TTF-1 is selectively expressed in the majority of schwannomas, particularly the conventional variants. Based on this observation, the TTF-1 protein and mRNA are specifically expressed in schwannomas. This highly aberrant expression of varying amounts of TTF-1 may provide new clues to reveal the pathogenesis of schwannoma.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Biomarcadores Tumorais / Proteínas de Ligação a DNA / Neurilemoma Tipo de estudo: Observational_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Hum Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Biomarcadores Tumorais / Proteínas de Ligação a DNA / Neurilemoma Tipo de estudo: Observational_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Hum Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2018 Tipo de documento: Article