Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research.
Sci Rep
; 7(1): 14641, 2017 11 07.
Article
em En
| MEDLINE
| ID: mdl-29116243
ABSTRACT
Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo. In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo, and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Placa Viral
/
Replicação Viral
/
Vírus Chikungunya
/
Aedes
/
Febre de Chikungunya
Tipo de estudo:
Evaluation_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Reino Unido