A recombinant BBSome core complex and how it interacts with ciliary cargo.
Elife
; 62017 11 15.
Article
em En
| MEDLINE
| ID: mdl-29168691
ABSTRACT
Cilia are small, antenna-like structures on the surface of eukaryotic cells that harbor a unique set of sensory proteins, including GPCRs and other membrane proteins. The transport of these proteins involves the BBSome, an eight-membered protein complex that is recruited to ciliary membranes by the G-protein Arl6. BBSome malfunction leads to Bardet-Biedl syndrome, a ciliopathy with severe consequences. Short ciliary targeting sequences (CTS) have been identified that trigger the transport of ciliary proteins. However, mechanistic studies that relate ciliary targeting to BBSome binding are missing. Here we used heterologously expressed BBSome subcomplexes to analyze the complex architecture and to investigate the binding of GPCRs and other receptors to the BBSome. A stable heterohexameric complex was identified that binds to GPCRs with interactions that only partially overlap with previously described CTS, indicating a more complex recognition than anticipated. Arl6â¢GTP does not affect these interactions, suggesting no direct involvement in cargo loading/unloading.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexos Multiproteicos
/
Multimerização Proteica
/
Proteínas Associadas aos Microtúbulos
Limite:
Humans
Idioma:
En
Revista:
Elife
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Alemanha