[Expression of XPG Gene in Forensic Age Estimation].
Fa Yi Xue Za Zhi
; 32(6): 415-419, 2016 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-29205966
ABSTRACT
OBJECTIVES:
To explore the expression of xeroderma pigmentosum complementation group G ï¼XPGï¼ gene in healthy Han population of different ages and to analysis the relationship between the mRNA and protein expression levels of XPG and age, which may provide a new molecular-biological indicator for forensic age determination.METHODS:
Total 150 samples of peripheral blood were collected from healthy Han population of different ages. Total RNA of peripheral blood mononuclear cell ï¼PBMCï¼ were extracted by TRIzol method, and the relative expression of XPG mRNA in PBMC was detected by quantitative real-time PCR, and the protein expression levels of XPG in plasma were detected by ELISA.RESULTS:
The mRNA and protein expression levels of XPG in ≤18 years old group were significantly different from 19-45 years old group and ≥46 years old group ï¼P<0.05ï¼, while there was no significant difference between 19-45 years old group and ≥46 years old group ï¼P>0.05ï¼. No significant sex differences were observed in mRNA and protein expression levels of XPG ï¼P>0.05ï¼.CONCLUSIONS:
The relative expression level of XPG mRNA in PBMC declines with the increase of age in younger age, while the protein expression level in plasma increases with age, and XPG gene can be used as one of new markers for forensic age estimation.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Proteínas Nucleares
/
Fatores Etários
/
Proteínas de Ligação a DNA
/
Endonucleases
Limite:
Adult
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Humans
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Middle aged
Idioma:
Zh
Revista:
Fa Yi Xue Za Zhi
Assunto da revista:
JURISPRUDENCIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China