Expression of fibronectin-binding protein of L. acidophilus NCFM and in vitro refolding to adhesion capable native-like protein from inclusion bodies.

Bisht, Sonu; Singh, Kumar Siddharth; Choudhary, Ritu; Kumar, Sudarshan; Grover, Sunita; Mohanty, Ashok Kumar; Pande, Veena; Kaushik, Jai Kumar

Bisht, Sonu; Singh, Kumar Siddharth; Choudhary, Ritu; Kumar, Sudarshan; Grover, Sunita; Mohanty, Ashok Kumar; Pande, Veena; Kaushik, Jai Kumar.

Afiliação

Bisht S; Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India; Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand, India.
Singh KS; Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India.
Choudhary R; Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India.
Kumar S; Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India.
Grover S; Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal 132001, India.
Mohanty AK; Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India.
Pande V; Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand, India.
Kaushik JK; Animal Biotechnology Centre, ICAR-National Dairy Research Institute, Karnal 132001, India. Electronic address: jai.kaushik@icar.gov.in.

Protein Expr Purif ; 145: 7-13, 2018 05.

Article em En | MEDLINE | ID: mdl-29229289

Assuntos

Adesinas Bacterianas/genética; Clonagem Molecular; Mucosa Intestinal; Lactobacillus acidophilus/metabolismo; Adesinas Bacterianas/química; Adesinas Bacterianas/isolamento & purificação; Adesinas Bacterianas/metabolismo; Escherichia coli/genética; Expressão Gênica; Humanos; Corpos de Inclusão; Redobramento de Proteína; Proteínas Recombinantes de Fusão/química; Proteínas Recombinantes de Fusão/isolamento & purificação; Proteínas Recombinantes de Fusão/metabolismo

Palavras-chave

Circular dichroism; Fibronectin/fibrinogen-binding protein; Host-microbe interactions; In vitro refolding; Inclusion bodies; Probiotic surface adhesins

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Clonagem Molecular / Adesinas Bacterianas / Mucosa Intestinal / Lactobacillus acidophilus Limite: Humans Idioma: En Revista: Protein Expr Purif Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Índia

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