A novel personalized 3D injectable protein scaffold for regenerative medicine.
J Mater Sci Mater Med
; 29(1): 7, 2017 Dec 14.
Article
em En
| MEDLINE
| ID: mdl-29243192
ABSTRACT
Biomaterials should be designed to closely resemble the characteristics and functions of the native extracellular matrix to provide mechanical support and signals to direct biological events. Here we have developed a novel injectable plasma rich in growth factors (PRGF-Endoret)-based formulation that combines a thermal-denaturation step of plasma with an autologous fibrin crosslinking. Rheological and mechanical properties were evaluated. Additionally, the microstructure and biological capacity of the biomaterial was also characterized. This novel formulation exhibited ideal mechanical properties and a gel-like behavior with the ability to progressively release its growth factor load over time. The results also suggested that the novel injectable formulation is non-cytotoxic, biocompatible and suitable for cell ingrowth as it is deduced from the fibroblast proliferation within the scaffold. Finally, stimulation of both cell proliferation and matrix proteins synthesis demonstrated the regenerative potential of this autologous protein based injectable scaffold.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
Fibrina
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Engenharia Tecidual
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Medicina Regenerativa
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Alicerces Teciduais
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Medicina de Precisão
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Mater Sci Mater Med
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Espanha