[Adsorption characteristics of proteins on membrane surface and effect of protein solution environment on permeation behavior of berberine].
Zhongguo Zhong Yao Za Zhi
; 42(20): 3912-3918, 2017 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-29243427
ABSTRACT
In order to explore the adsorption characteristics of proteins on the membrane surface and the effect of protein solution environment on the permeation behavior of berberine, berberine and proteins were used as the research object to prepare simulated solution. Low field NMR, static adsorption experiment and membrane separation experiment were used to study the interaction between the proteins and ceramic membrane or between the proteins and berberine. The static adsorption capacity of proteins, membrane relative flux, rejection rate of proteins, transmittance rate of berberine and the adsorption rate of proteins and berberine were used as the evaluation index. Meanwhile, the membrane resistance distribution, the particle size distribution and the scanning electron microscope (SEM) were determined to investigate the adsorption characteristics of proteins on ceramic membrane and the effect on membrane separation process of berberine. The results showed that the ceramic membrane could adsorb the proteins and the adsorption model was consistent with Langmuir adsorption model. In simulating the membrane separation process, proteins were the main factor to cause membrane fouling. However, when the concentration of proteins was 1 gâ¢L⻹, the proteins had no significant effect on membrane separation process of berberine.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Berberina
/
Proteínas
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Assunto da revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China