Harmine promotes molar root development via SMAD1/5/8 phosphorylation.
Biochem Biophys Res Commun
; 497(3): 924-929, 2018 03 11.
Article
em En
| MEDLINE
| ID: mdl-29253570
ABSTRACT
Tooth and bone are major tissues involved in physiological calcification in the body, and they use similar molecular pathways for development, homeostasis, and regeneration. Harmine (HMN) is a natural small compound that stimulates osteoblast differentiation in vitro and in vivo. Here we examined the biological effect of HMN on the postnatal development of molar tooth roots and periodontal tissues. HMN supported the formation of tooth roots and periodontal tissues in developing tooth germs. In tooth germ organ culture, HMN promoted the elongation of Hertwig's epithelial root sheath (HERS) and stimulated cell proliferation in HERS and dental follicle-derived tissues, including dental papillae and dental follicles. HMN stimulated cell proliferation and cell movement of HERS-derived cells without mesenchymal cells in vitro and directly induced the phosphorylation of SMAD1/5/8 protein in HERS-derived cells. Our results indicated that HMN was the first natural small compound to stimulate postnatal development of tooth germs.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosforilação
/
Raiz Dentária
/
Proteína Smad1
/
Proteína Smad5
/
Proteína Smad8
/
Harmina
/
Dente Molar
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão