Biochemical characterization of Campylobacter jejuni PNPase, an exoribonuclease important for bacterial pathogenicity.
Biochimie
; 147: 70-79, 2018 Apr.
Article
em En
| MEDLINE
| ID: mdl-29339148
ABSTRACT
Bacteria need to promptly respond to environmental changes. Ribonucleases (RNases) are key factors in the adaptation to new environments by enabling a rapid adjustment in RNA levels. The exoribonuclease polynucleotide phosphorylase (PNPase) is essential for low-temperature cell survival, affects the synthesis of proteins involved in virulence and has an important role in swimming, cell adhesion/invasion ability, and chick colonization in C. jejuni. However, the mechanism of action of this ribonuclease is not yet known. In this work we have characterized the biochemical activity of C. jejuni PNPase. Our results demonstrate that Cj-PNP is a processive 3' to 5' exoribonuclease that degrades single-stranded RNAs. Its activity is regulated according to the temperature and divalent ions. We have also shown that the KH and S1 domains are important for trimerization, RNA binding, and, consequently, for the activity of Cj-PNP. These findings will be helpful to develop new strategies for fighting against C. jejuni and may be extrapolated to other foodborne pathogens.
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Base de dados:
MEDLINE
Assunto principal:
Campylobacter jejuni
/
Exorribonucleases
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biochimie
Ano de publicação:
2018
Tipo de documento:
Article