The Pseudomonas aeruginosa ExoY phenotype of high-copy-number recombinants is not detectable in natural isolates.
Open Biol
; 8(1)2018 01.
Article
em En
| MEDLINE
| ID: mdl-29386405
ABSTRACT
The nucleotidyl cyclase ExoY is an effector protein of the type III secretion system of Pseudomonas aeruginosa We compared the cyclic nucleotide production and lung disease phenotypes caused by the ExoY-overexpressing strain PA103ΔexoUexoTTc pUCPexoY, its vector control strain PA103ΔexoUexoTTc pUCP18, its loss-of-function control PA103ΔexoUexoTTc pUCPexoY K81M and natural ExoY-positive and ExoY-negative isolates in a murine acute airway infection model. Only the P. aeruginosa carrier of the exoY-plasmid produced high levels of cUMP and caused the most severe course of infection. The pathology ascribed to ExoY from studies using the high-copy-number plasmid on mammalian cells in vitro and in vivo was not observed with natural P. aeruginosa isolates. This indicates that the role of ExoY during infection with real-life P. aeruginosa still needs to be resolved.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Fenótipo
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Pseudomonas aeruginosa
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Infecções por Pseudomonas
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Proteínas de Bactérias
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Dosagem de Genes
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Glucosiltransferases
Limite:
Animals
Idioma:
En
Revista:
Open Biol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Alemanha