Interactions of hyaluronan with oppositely charged surfactants in very diluted solutions in water.
Int J Biol Macromol
; 112: 241-249, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29391230
ABSTRACT
The phase behavior of aqueous systems containing hyaluronan, at concentrations between 2 and 100â¯mg/L, and oppositely charged surfactants was investigated. A fluorescence probe technique revealed the formation of micellar structures on the hyaluronan in homogeneous systems well below the surfactant standard, critical, micellar concentration. Moreover, regions of gel-phase separation were revealed. A detailed phase diagram was, thus, constructed in the very diluted region and the hyaluronan concentration was found to be the main parameter controlling the phase behavior, in contrast to the charge ratio. The stability of hyaluronan-surfactant aggregates in the homogeneous systems while in storage at 4⯰C (up to three months), against dilution, salt addition and on heating-cooling (between 10 and 50⯰C) was also investigated. The aggregates were stable while in storage or upon increasing and decreasing the temperature. The dilution of hyaluronan-surfactant complexes or the addition of 0.15â¯M NaCl led to their disintegration. Finally, systems prepared in a 0.15â¯M NaCl solution showed that interactions are suppressed and no aggregation below the standard critical micellar concentration was observed.
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Base de dados:
MEDLINE
Assunto principal:
Tensoativos
/
Água
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Ácido Hialurônico
Tipo de estudo:
Health_economic_evaluation
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
República Tcheca