Screening for Selective Protein Inhibitors by Using the IANUS Peptide Array.

Erdmann, Frank; Prell, Erik; Jahreis, Günther; Fischer, Gunter; Malesevic, Miroslav

Erdmann, Frank; Prell, Erik; Jahreis, Günther; Fischer, Gunter; Malesevic, Miroslav.

Afiliação

Erdmann F; Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120, Halle/Saale, Germany.
Prell E; Martin-Luther-University Halle-Wittenberg, Institute of Pharmacy, Department of Pharmacology, Wolfgang-Langenbeck-Strasse 4, 06120, Halle, Germany.
Jahreis G; Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120, Halle/Saale, Germany.
Fischer G; Present address: University Hospital Halle, Department for Radiation Medicine, Nuclear Medicine, Radiopharmacy, Ernst-Grube-Strasse 40, 06120, Halle, Germany.
Malesevic M; Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120, Halle/Saale, Germany.

Chembiochem ; 19(8): 789-792, 2018 04 16.

Article em En | MEDLINE | ID: mdl-29411932

ABSTRACT

ABSTRACT

Finding new road blacks A peptidic inhibitor of calcineurin (CaN)-mediated nuclear factor of activated Tâcells (NFAT) dephosphorylation, which is developed through a template-assisted IANUS (Induced orgANisation of strUcture by matrix-assisted togethernesS) peptide array, is cell permeable and able to block the translocation of green fluorescent protein-NFAT fusion protein (GFP-NFAT) into the nucleus after stimulation.

Assuntos

Inibidores de Calcineurina/farmacologia; Animais; Núcleo Celular/metabolismo; Proteínas de Fluorescência Verde/metabolismo; Humanos; Células Jurkat; Fatores de Transcrição NFATC/metabolismo; Fosforilação

Palavras-chave

calcineurin; fluorescence; inhibitors; microarrays; peptides; protein-protein interactions

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Inibidores de Calcineurina Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Revista: Chembiochem Assunto da revista: BIOQUIMICA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Alemanha

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