Mechanism of Stx17 recruitment to autophagosomes via IRGM and mammalian Atg8 proteins.
J Cell Biol
; 217(3): 997-1013, 2018 03 05.
Article
em En
| MEDLINE
| ID: mdl-29420192
Autophagy is a conserved eukaryotic process with metabolic, immune, and general homeostatic functions in mammalian cells. Mammalian autophagosomes fuse with lysosomes in a SNARE-driven process that includes syntaxin 17 (Stx17). How Stx17 translocates to autophagosomes is unknown. In this study, we show that the mechanism of Stx17 recruitment to autophagosomes in human cells entails the small guanosine triphosphatase IRGM. Stx17 directly interacts with IRGM, and efficient Stx17 recruitment to autophagosomes requires IRGM. Both IRGM and Stx17 directly interact with mammalian Atg8 proteins, thus being guided to autophagosomes. We also show that Stx17 is significant in defense against infectious agents and that Stx17-IRGM interaction is targeted by an HIV virulence factor Nef.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao GTP
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Proteínas Qa-SNARE
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Autofagossomos
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Família da Proteína 8 Relacionada à Autofagia
Limite:
Humans
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2018
Tipo de documento:
Article