An Attractive Reelin Gradient Establishes Synaptic Lamination in the Vertebrate Visual System.
Neuron
; 97(5): 1049-1062.e6, 2018 03 07.
Article
em En
| MEDLINE
| ID: mdl-29429939
ABSTRACT
A conserved organizational and functional principle of neural networks is the segregation of axon-dendritic synaptic connections into laminae. Here we report that targeting of synaptic laminae by retinal ganglion cell (RGC) arbors in the vertebrate visual system is regulated by a signaling system relying on target-derived Reelin and VLDLR/Dab1a on the projecting neurons. Furthermore, we find that Reelin is distributed as a gradient on the target tissue and stabilized by heparan sulfate proteoglycans (HSPGs) in the extracellular matrix (ECM). Through genetic manipulations, we show that this Reelin gradient is important for laminar targeting and that it is attractive for RGC axons. Finally, we suggest a comprehensive model of synaptic lamina formation in which attractive Reelin counter-balances repulsive Slit1, thereby guiding RGC axons toward single synaptic laminae. We establish a mechanism that may represent a general principle for neural network assembly in vertebrate species and across different brain areas.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Ganglionares da Retina
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Sinapses
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Vias Visuais
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Serina Endopeptidases
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Moléculas de Adesão Celular Neuronais
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Proteínas da Matriz Extracelular
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Rede Nervosa
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Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
Neuron
Assunto da revista:
NEUROLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
França