The effect of foxp3-overexpressing Treg cells on non-small cell lung cancer cells.
Mol Med Rep
; 17(4): 5860-5868, 2018 04.
Article
em En
| MEDLINE
| ID: mdl-29436663
ABSTRACT
The aim of the present study was to investigate the novel mechanisms of forkhead box protein P3 (foxp3) in T regulatory (Treg) cells in lung cancer behavior. Treg cells were isolated from the peripheral blood of healthy volunteers and then cocultured with 95D cells. A plasmid overexpressing foxp3 was constructed and transfected into Treg cells and an MTS assay was performed to assess cell viability. Flow cytometry was performed to evaluate cell apoptosis and reverse transcriptionquantitative polymerase chain reaction was used to measure mRNA expression. A Transwell assay was used to assess cell invasion. Treg cells were successfully isolated from peripheral blood with purity of 94.26%. Foxp3 expression in Treg cells was significantly increased following coculture with 95D cells, while matrix metalloproteinase9 expression was upregulated in 95D cells cocultured with Treg cells. The apoptosis, invasion and migration abilities of 95D cells were suppressed by coculture with Treg cells, whereas the adhesive ability was enhanced. Foxp3 overexpression in Treg cells enhanced the viability and invasiveness of 95D cells, whereas cell adhesion and migration were decreased. The results of the present study demonstrate that the viability and invasiveness of 95D cells are enhanced by foxp3 overexpression in Treg cells, indicating that increased levels of foxp3 in the tumor microenvironment may promote tumor cell growth.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
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Linfócitos T Reguladores
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Carcinoma Pulmonar de Células não Pequenas
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Fatores de Transcrição Forkhead
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Neoplasias Pulmonares
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2018
Tipo de documento:
Article