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Separation and Purification of Fructo-Oligosaccharide by High-Speed Counter-Current Chromatography Coupled with Precolumn Derivatization.
Duan, Wenjuan; Ji, Wenhua; Wei, Yuanan; Zhao, Ruixuan; Chen, Zijian; Geng, Yanling; Jing, Feng; Wang, Xiao.
Afiliação
  • Duan W; Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of TCM Quality Control Technology, 19 Keyuan Street, Jinan 250014, Shandong, China. duanwj4048@126.com.
  • Ji W; Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of TCM Quality Control Technology, 19 Keyuan Street, Jinan 250014, Shandong, China. jwh519@163.com.
  • Wei Y; Quantum Hi-Tech (China) Biological Co., Ltd., 133 Gaoxin Road West, Hi-tech Zone, Jiangmen 529081, Guangdong, China. yawei@qht.cc.
  • Zhao R; Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of TCM Quality Control Technology, 19 Keyuan Street, Jinan 250014, Shandong, China. zhaoruixuan2016@126.com.
  • Chen Z; Quantum Hi-Tech (China) Biological Co., Ltd., 133 Gaoxin Road West, Hi-tech Zone, Jiangmen 529081, Guangdong, China. chenzj@qht.cc.
  • Geng Y; Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of TCM Quality Control Technology, 19 Keyuan Street, Jinan 250014, Shandong, China. gengyanling@126.com.
  • Jing F; Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of TCM Quality Control Technology, 19 Keyuan Street, Jinan 250014, Shandong, China. jingfeng20052009@163.com.
  • Wang X; Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Shandong Key Laboratory of TCM Quality Control Technology, 19 Keyuan Street, Jinan 250014, Shandong, China. wxjn1998@126.com.
Molecules ; 23(2)2018 Feb 10.
Article em En | MEDLINE | ID: mdl-29439422
High-speed counter-current chromatography (HSCCC) coupled with precolumn derivatization was developed for isolating and purifying fructo-oligosaccharides (FOSs). Firstly, the total FOSs were precolumn derivatized and then separated by high-speed counter-current chromatography (HSCCC) with two-phase solvent system petroleum ether-n-butanol-methanol-water (3:2:1:4, v/v). Secondly, the obtained compounds were deacetylated and the fructo-oligosaccharides (FOSs) with high purity were obtained. Their structures were identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR). This research successfully established a novel strategy for separation and purification of FOS. There is no doubt that the application of the research will be beneficial for the quantitative and qualitative analysis of products containing FOSs.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligossacarídeos Tipo de estudo: Qualitative_research Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligossacarídeos Tipo de estudo: Qualitative_research Idioma: En Revista: Molecules Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: China