Podoplanin-positive myofibroblasts: a pathological hallmark of pleuroparenchymal fibroelastosis.
Histopathology
; 72(7): 1209-1215, 2018 Jun.
Article
em En
| MEDLINE
| ID: mdl-29468722
ABSTRACT
Pathological differential diagnoses of pleuroparenchymal fibroelastosis (PPFE) include usual interstitial pneumonia (UIP) and pulmonary apical cap (PAC); however, there are no specific immunostaining makers to distinguish between these diseases. We performed immunohistochemistry using several pleural mesothelial cell-related markers, including cytokeratin-5/6, CAM5.2, WT-1, calretinin, desmin and podoplanin, for PPFE (n = 4), UIP (n = 10) and PAC (n = 3) lung sections. Among the examined markers, in PPFE and PAC lungs podoplanin commonly showed positivity for spindle cells both in thickened pleura and subpleural fibroelastosis lesions; these cells were also stained with α-smooth muscle actin, a marker of myofibroblasts. However, even in elastic fibre-rich cases, UIP lungs did not show such podoplanin-positive myofibroblasts in pleura/subpleura and fibroblastic foci. These findings were also verified using immunofluorescence. By contrast, immunohistochemically as well as morphologically, the difference between PPFE and PAC was not apparent. The presence of podoplanin-positive myofibroblasts could be a pathological hallmark of PPFE, suggesting a pathogenic process distinct from UIP but common to PAC.
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Base de dados:
MEDLINE
Assunto principal:
Doenças Pulmonares Intersticiais
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Fibrose Pulmonar Idiopática
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Miofibroblastos
Tipo de estudo:
Diagnostic_studies
Limite:
Aged
/
Female
/
Humans
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Male
/
Middle aged
Idioma:
En
Revista:
Histopathology
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão