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Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK ß-Subunit Carbohydrate-Binding Modules.
Gooley, Paul R; Koay, Ann; Mobbs, Jesse I.
Afiliação
  • Gooley PR; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. prg@unimelb.edu.au.
  • Koay A; Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.
  • Mobbs JI; Experimental Therapeutics Centre, Agency for Science Technology and Research, Singapore, Singapore.
Methods Mol Biol ; 1732: 87-98, 2018.
Article em En | MEDLINE | ID: mdl-29480470
Understanding the kinetics of proteins interacting with their ligands is important for characterizing molecular mechanism. However, it can be difficult to determine the extent and nature of these interactions for weakly formed protein-ligand complexes that have lifetimes of micro- to milliseconds. Nuclear magnetic resonance (NMR) spectroscopy is a powerful solution-based method for the atomic-level analysis of molecular interactions on a wide range of timescales, including micro- to milliseconds. Recently the combination of thermodynamic experiments using isothermal titration calorimetry (ITC) with kinetic measurements using ZZ-exchange and CPMG relaxation dispersion NMR spectroscopy have been used to determine the kinetics of weakly interacting protein systems. This chapter describes the application of ITC and NMR to understand the differences in the kinetics of carbohydrate binding by the ß1- and ß2-carbohydrate-binding modules of AMP-activated protein kinase.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Calorimetria / Carboidratos / Modelos Moleculares / Ressonância Magnética Nuclear Biomolecular / Proteínas Quinases Ativadas por AMP Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Austrália

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Calorimetria / Carboidratos / Modelos Moleculares / Ressonância Magnética Nuclear Biomolecular / Proteínas Quinases Ativadas por AMP Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Austrália