A quantitative method for staging mouse embryos based on limb morphometry.
Development
; 145(7)2018 04 05.
Article
em En
| MEDLINE
| ID: mdl-29540505
ABSTRACT
To determine the developmental stage of embryonic mice, we apply a geometric morphometric approach to the changing shape of the mouse limb bud as it grows from embryonic day 10 to embryonic day 15 post-conception. As the ontogenetic sequence results in the de novo emergence of shape features not present in the early stages, we have created a standard ontogenetic trajectory for limb bud development - a quantitative characterization of shape change during limb morphogenesis. This trajectory of form as a function of time also gives us the reverse function the ability to infer developmental stage from form, with a typical uncertainty of 2â
h. We introduce eMOSS (embryonic mouse ontogenetic staging system) as a fast, reliable, convenient and freely available online tool for staging embryos from two-dimensional images of their limb buds, and illustrate its use in phenotyping early limb abnormalities.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Idade Gestacional
/
Botões de Extremidades
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Embrião de Mamíferos
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Membro Posterior
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Morfogênese
Limite:
Animals
Idioma:
En
Revista:
Development
Assunto da revista:
BIOLOGIA
/
EMBRIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article