Toosendanin inhibits adipogenesis by activating Wnt/ß-catenin signaling.
Sci Rep
; 8(1): 4626, 2018 03 15.
Article
em En
| MEDLINE
| ID: mdl-29545541
ABSTRACT
Toosendanin (TSN), a triterpenoid extracted from Melia toosendan, has been reported to possess anti-oxidant, anti-inflammatory, anti-allergic, and anti-arthritic activities. However, its anti-adipogenic effect remains unknown. Here, we found that TSN dose-dependently attenuated lipid accumulation in preadipocytes 3T3-L1 as evidenced by Oil Red O staining. TSN also significantly downregulated mRNA and protein levels of adipocytokines (adiponectin and leptin), CCAAT/enhancer binding proteins α (C/EBP-α), peroxisome proliferator-activated receptor γ (PPAR-γ), fatty acid synthase, and acetyl-CoA carboxylase in adipocytes. To understand the mechanism, we observed that TSN effectively activated Wnt/ß-catenin pathway, in which TSN increased low density lipoprotein receptor related protein 6, disheveled 2, ß-catenin, and cyclin D1 expression levels, while it inactivated glycogen synthase kinase 3ß by enhancing its phosphorylation. Moreover, TSN reduced weight of gonadal white fat and serum triacylglycerol (TAG) content in high-fat diet (HFD)-fed mice. Interestingly, the in vivo studies also demonstrated that TSN promoted the expression of ß-catenin, but accordingly repressed C/EBP-α and PPAR-γ expression in HFD-induced mice. Overall, TSN is capable of inhibiting the lipogenesis of adipocytes by activating the Wnt/ß-catenin pathway, suggesting potential application of TSN as a natural anti-obesity agent.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Regulação da Expressão Gênica
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Fármacos Antiobesidade
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Proteínas Wnt
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Beta Catenina
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Adipogenia
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Obesidade
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China