Coordinated Activity of Y Family TLS Polymerases and EXO1 Protects Non-S Phase Cells from UV-Induced Cytotoxic Lesions.
Mol Cell
; 70(1): 34-47.e4, 2018 04 05.
Article
em En
| MEDLINE
| ID: mdl-29551515
ABSTRACT
UV-induced photoproducts are responsible for the pathological effects of sunlight. Mutations in nucleotide excision repair (NER) cause severe pathologies characterized by sunlight sensitivity, coupled to elevated predisposition to cancer and/or neurological dysfunctions. We have previously shown that in UV-irradiated non-cycling cells, only a particular subset of lesions activates the DNA damage response (DDR), and this requires NER and EXO1 activities. To define the molecular mechanism acting at these lesions, we demonstrate that Y family TLS polymerases are recruited at NER- and EXO1-positive lesion sites in non-S phase cells. The coordinated action of EXO1 and Y family TLS polymerases promotes checkpoint activation, leads to lesion repair, and is crucial to prevent cytotoxic double-strand break (DSB) formation.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
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Enzimas Reparadoras do DNA
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DNA Polimerase Dirigida por DNA
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Reparo do DNA
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Exodesoxirribonucleases
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Quebras de DNA de Cadeia Dupla
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Pontos de Checagem do Ciclo Celular
Limite:
Humans
Idioma:
En
Revista:
Mol Cell
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2018
Tipo de documento:
Article