Microglia Analysis in Retinal Degeneration Mouse Models.
Methods Mol Biol
; 1753: 159-166, 2018.
Article
em En
| MEDLINE
| ID: mdl-29564787
ABSTRACT
Microgliosis is a hallmark of degenerative processes in the retina. Reactive microglia migrate to the photoreceptor layer and the subretinal space during outer retinal degeneration. This process creates a toxic milieu where reactive microglia and dying photoreceptors recruit additional reactive phagocytes. This results in the release of a multitude of proinflammatory factors which accelerate photoreceptor demise. In this chapter, we outline in detail how to monitor microgliosis in the Fam161a-deficient mouse model of Retinitis Pigmentosa by performing immunohistochemical stainings of retinal cryosections and flat mounts using the marker Iba1. This protocol will serve as a guideline in evaluating microglia reactivity and localization in various mouse models of retinal degeneration.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Fotorreceptoras
/
Imuno-Histoquímica
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Retinose Pigmentar
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Microglia
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Alemanha