Your browser doesn't support javascript.
loading
CNS myeloid cells critically regulate heat hyperalgesia.
Kälin, Stefanie; Miller, Kelly R; Kälin, Roland E; Jendrach, Marina; Witzel, Christian; Heppner, Frank L.
Afiliação
  • Kälin S; Department of Neuropathology and.
  • Miller KR; Department of Neuropathology and.
  • Kälin RE; Department of Neuropathology and.
  • Jendrach M; Department of Neuropathology and.
  • Witzel C; Department of Plastic Surgery, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
  • Heppner FL; Department of Neuropathology and.
J Clin Invest ; 128(7): 2774-2786, 2018 07 02.
Article em En | MEDLINE | ID: mdl-29634489
ABSTRACT
Activation of non-neuronal microglia is thought to play a causal role in spinal processing of neuropathic pain. To specifically investigate microglia-mediated effects in a model of neuropathic pain and overcome the methodological limitations of previous approaches exploring microglia function upon nerve injury, we selectively ablated resident microglia by intracerebroventricular ganciclovir infusion into male CD11b-HSVTK-transgenic mice, which was followed by a rapid, complete, and persistent (23 weeks) repopulation of the CNS by peripheral myeloid cells. In repopulated mice that underwent sciatic nerve injury, we observed a normal response to mechanical stimuli, but an absence of thermal hypersensitivity ipsilateral to the injured nerve. Furthermore, we found that neuronal expression of calcitonin gene-related peptide (CGRP), which is a marker of neurons essential for heat responses, was diminished in the dorsal horn of the spinal cord in repopulated mice. These findings identify distinct mechanisms for heat and mechanical hypersensitivity and highlight a crucial contribution of CNS myeloid cells in the facilitation of noxious heat.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistema Nervoso Central / Células Mieloides / Hiperalgesia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Clin Invest Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistema Nervoso Central / Células Mieloides / Hiperalgesia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Clin Invest Ano de publicação: 2018 Tipo de documento: Article