Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics.
Science
; 360(6391)2018 05 25.
Article
em En
| MEDLINE
| ID: mdl-29674432
ABSTRACT
Flatworms of the species Schmidtea mediterranea are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate into all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterized gene expression changes during differentiation and discovered cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Planárias
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Atlas como Assunto
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Células
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Linhagem da Célula
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Perfilação da Expressão Gênica
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Análise de Célula Única
Limite:
Animals
Idioma:
En
Revista:
Science
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Alemanha