miRNAs in platelet-poor blood plasma and purified RNA are highly stable: a confirmatory study.
BMC Res Notes
; 11(1): 273, 2018 May 04.
Article
em En
| MEDLINE
| ID: mdl-29728133
ABSTRACT
OBJECTIVE:
We wished to re-assess the relative stability of microRNAs (miRNAs) as compared with other RNA molecules, which has been confirmed in many contexts. When bound to Argonaute proteins, miRNAs are protected from degradation, even when released into the extracellular space in ribonucleoprotein complexes, and with or without the protection of membranes in extracellular vesicles. Purified miRNAs also appear to present less of a target for degradation than other RNAs. Although miRNAs are by no means immune to degradation, biological samples subjected to prolonged incubation at room temperature, multiple freeze/thaws, or collection in the presence of inhibitors like heparin, can typically be remediated or used directly for miRNA measurements.RESULTS:
Here, we provide additional confirmation of early, well validated findings on miRNA stability and detectability. Our data also suggest that inadequate depletion of platelets from plasma may explain the occasional report that freeze-thaw cycles can adversely affect plasma miRNA levels. Overall, the repeated observation of miRNA stability is again confirmed.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Preservação Biológica
/
Preservação de Tecido
/
Plaquetas
/
Estabilidade de RNA
/
MicroRNAs
/
Proteínas Argonautas
Limite:
Humans
Idioma:
En
Revista:
BMC Res Notes
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos