UHPLC-QqQ-MS/MS identification, quantification of polyphenols from Passiflora subpeltata fruit pulp and determination of nutritional, antioxidant, α-amylase and α-glucosidase key enzymes inhibition properties.
Food Res Int
; 108: 611-620, 2018 06.
Article
em En
| MEDLINE
| ID: mdl-29735097
ABSTRACT
The diabetic key enzymes inhibition, nutritional, antioxidant activity and bioactive compounds identification of Passiflora subpeltata fruit pulp were investigated. Fifteen polyphenolic compounds including protocatechuic acid, ferulic acid, vanillic acid, epicatechin, p-coumaric acid, cinnamic acid, eriodictyol and quercetin-3-glucoside were identified in the pulp of this species by using UHPLC-QqQ-MS/MS analysis. The total carbohydrates and crude protein contents in fruit pulp were 2.62â¯mg glucose equivalent/g sample fruit pulp and 8.80â¯mg BSA equivalent/g sample fruit pulp, respectively. The fresh fruit pulp of P. subpeltata contained high total phenolic (724.76â¯mg GAE/g sample) content and it revealed very high DPPH⢠(IC50 of 5.667⯵g/mL) and ABTS+⢠(6794.96⯵M trolox equivalent/g sample) scavenging activities. In the key enzymes assays useful for diabetic inhibition the fresh fruit pulp characterized maximum inhibition of α-amylase and α-glucosidase IC50 of 18.69 and 32.63⯵g/mL, respectively. Thus, these results lead to conclude that this fruit specie could be very useful source in nutraceutical products preparations for Type 2 diabetic suffering humans.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
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Espectrometria de Massas por Ionização por Electrospray
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Passiflora
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Alfa-Amilases
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Espectrometria de Massas em Tandem
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Polifenóis
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Inibidores de Glicosídeo Hidrolases
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Análise de Alimentos
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Manipulação de Alimentos
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Frutas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Food Res Int
Ano de publicação:
2018
Tipo de documento:
Article