Global methylation profiles in buccal cells of long-term smokers and moist snuff consumers.
Biomarkers
; 23(7): 625-639, 2018 Nov.
Article
em En
| MEDLINE
| ID: mdl-29771158
ABSTRACT
PURPOSE:
Alternations in gene methylation and other epigenetic changes regulate normal development as well as drive disease progression. The aim of this study is to investigate global methylation changes in the buccal cells of smokers and smokeless tobacco users. MATERIALS ANDMETHODS:
Generally healthy adult male subjects were recruited into smoker (SMK), moist snuff consumer (MSC) and non-tobacco consumer (NTC) cohorts (40 subjects/cohort) (ClinicalTrials.gov Identifier NCT01923402). Global methylation profiling was performed on Illumina 450 K methylation arrays using buccal cell DNAs.RESULTS:
The SMK cohort exhibited larger qualitative and quantitative changes relative to MSC. Approximately half of the differentially methylated 1252 gene loci were grouped as combustible tobacco-related (CTR) signatures and a third of the changes, tobacco-related (TR) signatures, were associated with smoking. Very few (41) differentially methylated gene loci were exclusively associated with moist snuff use and were designated as moist snuff-related (MSR) signature. Pathway enrichment analyses revealed that developmental and immune response pathways, among others, were impacted due to tobacco use.CONCLUSIONS:
Chronic cigarette smoking causes hyper- and hypo-methylation of genes that could contribute to smoking-related diseases. These results help place combustible and non-combustible tobacco products along a risk continuum and provide additional insights into the effects of tobacco consumption.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bochecha
/
Tabaco sem Fumaça
/
Metilação de DNA
/
Fumantes
Tipo de estudo:
Etiology_studies
/
Observational_studies
/
Prevalence_studies
/
Qualitative_research
/
Risk_factors_studies
Limite:
Adult
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Biomarkers
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos