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Loss of TIGAR Induces Oxidative Stress and Meiotic Defects in Oocytes from Obese Mice.
Wang, Haichao; Cheng, Qing; Li, Xiaoyan; Hu, Feifei; Han, Longsen; Zhang, Hao; Li, Ling; Ge, Juan; Ying, Xiaoyan; Guo, Xuejiang; Wang, Qiang.
Afiliação
  • Wang H; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University.
  • Cheng Q; §Departement of Obstetrics, Obstetrics and Gynecology Hospital Affiliated to Nanjing Medical University.
  • Li X; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University.
  • Hu F; ¶College of Animal Science & Technology, Nanjing Agricultural University.
  • Han L; ‖Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China, 210000.
  • Zhang H; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University.
  • Li L; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University.
  • Ge J; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University.
  • Ying X; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University.
  • Guo X; ‖Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China, 210000.
  • Wang Q; From the ‡State Key Laboratory of Reproductive Medicine, Nanjing Medical University; qwang2012@njmu.edu.cn guo_xuejiang@njmu.edu.cn.
Mol Cell Proteomics ; 17(7): 1354-1364, 2018 07.
Article em En | MEDLINE | ID: mdl-29776966
Maternal obesity has been reported to impair oocyte quality in mice, however, the underlying mechanisms remain unclear. In the present study, by conducting a comparative proteomic analysis, we identified a reduced expression of TIGAR (TP53-induced glycolysis and apoptosis regulator) protein in ovulated oocytes from high-fat diet (HFD)-fed mice. Specific depletion of TIGAR in mouse oocytes results in the marked elevation of reactive oxygen species (ROS) levels and the failure of meiotic apparatus assembly. Importantly, forced expression of TIGAR in HFD oocytes not only attenuates ROS production, but also partly prevents spindle disorganization and chromosome misalignment during meiosis. Meantime, we noted that TIGAR knockdown in oocytes induces a strong activation of autophagy, whereas overexpression of TIGAR significantly reduces the LC3 accumulation in HFD oocytes. By anti-oxidant treatment, we further demonstrated that such an autophagic response is dependent on the TIGAR-controlled ROS production. In summary, our data indicate a role for TIGAR in modulating redox homeostasis during oocyte maturation, and uncover that loss of TIGAR is a critical pathway mediating the effects of maternal obesity on oocyte quality.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oócitos / Proteínas / Estresse Oxidativo / Meiose Tipo de estudo: Guideline / Prognostic_studies Limite: Animals Idioma: En Revista: Mol Cell Proteomics Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oócitos / Proteínas / Estresse Oxidativo / Meiose Tipo de estudo: Guideline / Prognostic_studies Limite: Animals Idioma: En Revista: Mol Cell Proteomics Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2018 Tipo de documento: Article