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Automated, parallel mass spectrometry imaging and structural identification of lipids.
Ellis, Shane R; Paine, Martin R L; Eijkel, Gert B; Pauling, Josch K; Husen, Peter; Jervelund, Mark W; Hermansson, Martin; Ejsing, Christer S; Heeren, Ron M A.
Afiliação
  • Ellis SR; The Maastricht Multimodal Molecular Imaging Institute (M4I), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands.
  • Paine MRL; The Maastricht Multimodal Molecular Imaging Institute (M4I), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands.
  • Eijkel GB; The Maastricht Multimodal Molecular Imaging Institute (M4I), Division of Imaging Mass Spectrometry, Maastricht University, Maastricht, the Netherlands.
  • Pauling JK; Department of Biochemistry and Molecular Biology, Villum Center for Bioanalytical Sciences, University of Southern Denmark, Odense, Denmark.
  • Husen P; Theoretical Biophysics, Institute of Biology, Humboldt-Universität zu Berlin, Berlin, Germany.
  • Jervelund MW; Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense, Denmark.
  • Hermansson M; Department of Biochemistry and Molecular Biology, Villum Center for Bioanalytical Sciences, University of Southern Denmark, Odense, Denmark.
  • Ejsing CS; Department of Biochemistry and Molecular Biology, Villum Center for Bioanalytical Sciences, University of Southern Denmark, Odense, Denmark.
  • Heeren RMA; Department of Biochemistry and Molecular Biology, Villum Center for Bioanalytical Sciences, University of Southern Denmark, Odense, Denmark. cse@bmb.sdu.dk.
Nat Methods ; 15(7): 515-518, 2018 07.
Article em En | MEDLINE | ID: mdl-29786091
ABSTRACT
We report a method that enables automated data-dependent acquisition of lipid tandem mass spectrometry data in parallel with a high-resolution mass spectrometry imaging experiment. The method does not increase the total image acquisition time and is combined with automatic structural assignments. This lipidome-per-pixel approach automatically identified and validated 104 unique molecular lipids and their spatial locations from rat cerebellar tissue.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Automação / Espectrometria de Massas / Lipídeos Tipo de estudo: Diagnostic_studies Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Holanda
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Automação / Espectrometria de Massas / Lipídeos Tipo de estudo: Diagnostic_studies Idioma: En Revista: Nat Methods Assunto da revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Holanda