Fluorogenic Probing of Membrane Protein Trafficking.
Bioconjug Chem
; 29(6): 1823-1828, 2018 06 20.
Article
em En
| MEDLINE
| ID: mdl-29791141
ABSTRACT
Methods to differentially label cell-surface and intracellular membrane proteins are indispensable for understanding their function and the regulation of their trafficking. We present an efficient strategy for the rapid and selective fluorescent labeling of membrane proteins based on the chemical-genetic fluorescent marker FAST (fluorescence-activating and absorption-shifting tag). Cell-surface FAST-tagged proteins could be selectively and rapidly labeled using fluorogenic membrane-impermeant 4-hydroxybenzylidene rhodanine (HBR) analogs. This approach allows the study of protein trafficking at the plasma membrane with various fluorometric techniques, and opens exciting prospects for the high-throughput screening of small molecules able to restore disease-related trafficking defects.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rodanina
/
Compostos de Benzilideno
/
Membrana Celular
/
Corantes Fluorescentes
/
Proteínas de Membrana
Limite:
Humans
Idioma:
En
Revista:
Bioconjug Chem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
França