Bio-production of Baccatin III, an Important Precursor of Paclitaxel by a Cost-Effective Approach.
Mol Biotechnol
; 60(7): 492-505, 2018 Jul.
Article
em En
| MEDLINE
| ID: mdl-29796788
ABSTRACT
Natural production of anti-cancer drug taxol from Taxus has proved to be environmentally unsustainable and economically unfeasible. Currently, bioengineering the biosynthetic pathway of taxol is an attractive alternative production approach. 10-deacetylbaccatin III-10-O-acetyl transferase (DBAT) was previously characterized as an acyltransferase, using 10-deacetylbaccatin III (10-DAB) and acetyl CoA as natural substrates, to form baccatin III in the taxol biosynthesis. Here, we report that other than the natural acetyl CoA (Ac-CoA) substrate, DBAT can also utilize vinyl acetate (VA), which is commercially available at very low cost, acylate quickly and irreversibly, as acetyl donor in the acyl transfer reaction to produce baccatin III. Furthermore, mutants were prepared via a semi-rational design in this work. A double mutant, I43S/D390R was constructed to combine the positive effects of the different single mutations on catalytic activity, and its catalytic efficiency towards 10-DAB and VA was successfully improved by 3.30-fold, compared to that of wild-type DBAT, while 2.99-fold higher than the catalytic efficiency of WT DBAT towards 10-DAB and Ac-CoA. These findings can provide a promising economically and environmentally friendly method for exploring novel acyl donors to engineer natural product pathways.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetiltransferases
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Taxus
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Alcaloides
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Antineoplásicos Fitogênicos
Tipo de estudo:
Health_economic_evaluation
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Prognostic_studies
Idioma:
En
Revista:
Mol Biotechnol
Assunto da revista:
BIOLOGIA MOLECULAR
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BIOTECNOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article