The Rab GTPase activating protein Gyp2 contributes to UV stress tolerance in Metarhizium acridum.
World J Microbiol Biotechnol
; 34(6): 78, 2018 May 23.
Article
em En
| MEDLINE
| ID: mdl-29796938
ABSTRACT
GTPase activation protein (GAP) for Rab GTPases can accelerate GTP hydrolysis to alter the activity of Rab GTPases. To explore the function of GAP in entomopathogenic fungi, we constructed a deletion mutant of Gyp2 gene, a member of the Gyp (GAP for Ypt/Rab proteins) family in the locust-specific fungal pathogen, Metarhizium acridum. Results showed that the ∆MaGyp2 mutant had dramatically decreased tolerance to ultraviolet irradiation compared to wild type strain. Quantitative real-time PCR revealed that UV irradiation repair related genes Uve1 and WC1 were downregulated in ∆MaGyp2 mutant. Seven of other ten Gyp family members had significantly increased transcription in ∆MaGyp2 mutant compared with wild type, which may partly rescue the deficiency of MaGyp2.
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Base de dados:
MEDLINE
Assunto principal:
Tolerância a Radiação
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Raios Ultravioleta
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Proteínas Ativadoras de GTPase
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Metarhizium
Limite:
Animals
Idioma:
En
Revista:
World J Microbiol Biotechnol
Ano de publicação:
2018
Tipo de documento:
Article