In vitro method for 3D morphometry of human articular cartilage chondrons based on micro-computed tomography.
Osteoarthritis Cartilage
; 26(8): 1118-1126, 2018 08.
Article
em En
| MEDLINE
| ID: mdl-29802974
ABSTRACT
OBJECTIVE:
The aims of this study were to 1) develop a novel sample processing protocol to visualize human articular cartilage (AC) chondrons using micro-computed tomography (µCT), 2) develop and validate an algorithm to quantify the chondron morphology in 3D, and 3) compare the differences in chondron morphology between intact and osteoarthritic AC.METHOD:
The developed protocol is based on the dehydration of samples with hexamethyldisilazane (HMDS), followed by imaging with a desktop µCT. Chondron density and depth, as well as volume and sphericity, were calculated in 3D with a custom-made and validated algorithm employing semi-automatic chondron selection and segmentation. The quantitative parameters were analyzed at three AC depth zones (zone 1 0-10%; zone 2 10-40%; zone 3 40-100%) and grouped by the OARSI histological grades (OARSI grades 0-1.0, n = 6; OARSI grades 3.0-3.5, n = 6).RESULTS:
After semi-automatic chondron selection and segmentation, 1510 chondrons were approved for 3D morphometric analyses. The chondrons especially in the deeper tissue (zones 2 and 3) were significantly larger (P < 0.001) and less spherical (P < 0.001), respectively, in the OARSI grade 3-3.5 group compared to the OARSI grade 0-1.0 group. No statistically significant difference in chondron density between the OARSI grade groups was observed at different depths.CONCLUSION:
We have developed a novel sample processing protocol for chondron imaging in 3D, as well as a high-throughput algorithm to semi-automatically quantify chondron/chondrocyte 3D morphology in AC. Our results also suggest that 3D chondron morphology is affected by the progression of osteoarthritis (OA).Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cartilagem Articular
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Condrócitos
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Imageamento Tridimensional
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Microtomografia por Raio-X
Limite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Osteoarthritis Cartilage
Assunto da revista:
ORTOPEDIA
/
REUMATOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article