GateFinder: projection-based gating strategy optimization for flow and mass cytometry.
Bioinformatics
; 34(23): 4131-4133, 2018 12 01.
Article
em En
| MEDLINE
| ID: mdl-29850785
ABSTRACT
Motivation High-parameter single-cell technologies can reveal novel cell populations of interest, but studying or validating these populations using lower-parameter methods remains challenging. Results:
Here, we present GateFinder, an algorithm that enriches high-dimensional cell types with simple, stepwise polygon gates requiring only two markers at a time. A series of case studies of complex cell types illustrates how simplified enrichment strategies can enable more efficient assays, reveal novel biomarkers and clarify underlying biology. Availability and implementation The GateFinder algorithm is implemented as a free and open-source package for BioConductor https//nalab.stanford.edu/gatefinder. Supplementary information Supplementary data are available at Bioinformatics online.
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1
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
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Software
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Biomarcadores
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Citometria de Fluxo
Idioma:
En
Revista:
Bioinformatics
Assunto da revista:
INFORMATICA MEDICA
Ano de publicação:
2018
Tipo de documento:
Article