The p53-signaling pathway and colorectal cancer: Interactions between downstream p53 target genes and miRNAs.
Genomics
; 111(4): 762-771, 2019 07.
Article
em En
| MEDLINE
| ID: mdl-29860032
ABSTRACT
INTRODUCTION:
We examined expression of genes in the p53-signaling pathway. We determine if genes that have significantly different expression in carcinoma tissue compared to normal mucosa also have significantly differentially expressed miRNAs. We utilize a sample of 217 CRC cases.METHODS:
We focused on fold change (FC)â¯>â¯1.50 or <0.67 for genes and miRNAs, that were statistically significant after adjustment for multiple comparisons. We evaluated the linear association between the differential expression of miRNA and mRNA. miRNAmRNA seed-region matches also were determined.RESULTS:
Eleven dysregulated genes were associated with 37 dysregulated miRNAs; all were down-stream from the TP53 gene. MiR-150-5p (HRâ¯=â¯0.82) and miR-196b-5p (HR 0.73) significantly reduced the likelihood of dying from CRC when miRNA expression increased in rectal tumors.CONCLUSIONS:
Our data suggest that activation of p53 from cellular stress, could target downstream genes that in turn could influence cell cycle arrest, apoptosis, and angiogenesis through mRNAmiRNA interactions.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Carcinoma
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Neoplasias Colorretais
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Regulação Neoplásica da Expressão Gênica
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Proteína Supressora de Tumor p53
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MicroRNAs
Limite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2019
Tipo de documento:
Article