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Development of a continuous cell line from larval Atlantic cod (Gadus morhua) and its use in the study of the microsporidian, Loma morhua.
MacLeod, Michael J; Vo, Nguyen T K; Mikhaeil, Michael S; Monaghan, S Richelle; Alexander, J Andrew N; Saran, Mandeep K; Lee, Lucy E J.
Afiliação
  • MacLeod MJ; Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada.
  • Vo NTK; Department of Biology, University of Waterloo, Waterloo, ON, Canada.
  • Mikhaeil MS; Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada.
  • Monaghan SR; Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada.
  • Alexander JAN; Faculty of Science, University of the Fraser Valley, Abbotsford, BC, Canada.
  • Saran MK; Faculty of Science, University of the Fraser Valley, Abbotsford, BC, Canada.
  • Lee LEJ; Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada.
J Fish Dis ; 41(9): 1359-1372, 2018 Sep.
Article em En | MEDLINE | ID: mdl-29882595
ABSTRACT
In vitro cell culture methods are crucial for the isolation, purification and mass propagation of intracellular pathogens of aquatic organisms. Cell culture infection models can yield insights into infection mechanisms, aid in developing methods for disease mitigation and prevention, and inform commercial-scale cultivation approaches. This study details the establishment of a larval cell line (GML-5) from the Atlantic cod (Gadus morhua) and its use in the study of microsporidia. GML-5 has survived over 100 passages in 8 years of culture. The line remains active and viable between 8 and 21°C in Leibovitz-15 (L-15) media with 10% foetal bovine serum and exhibits a myofibroblast phenotype as indicated by immuno-positive results for vimentin, α-smooth muscle actin, collagen I and S-100 proteins, while being desmin-negative. GML-5 supports the infection and development of two microsporidian parasites, an opportunistic generalist (Anncaliia algerae) and cod-specific Loma morhua. Using GML-5, spore germination and proliferation of L. morhua was found to require exposure to basic pH and cool incubation temperatures (8°C), in contrast to A. algerae, which required no cultural modifications. Loma morhua-associated xenoma-like structures were observed 2 weeks postexposure. This in vitro infection model may serve as a valuable tool for cod parasitology and aquaculture research.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linhagem Celular / Gadus morhua / Técnicas de Cultura de Tecidos / Loma / Larva Limite: Animals Idioma: En Revista: J Fish Dis Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Linhagem Celular / Gadus morhua / Técnicas de Cultura de Tecidos / Loma / Larva Limite: Animals Idioma: En Revista: J Fish Dis Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Canadá