Dual-signal ratiometric electrochemiluminescence assay for detecting the activity of human methyltransferase.
Analyst
; 143(14): 3353-3359, 2018 Jul 09.
Article
em En
| MEDLINE
| ID: mdl-29893757
ABSTRACT
In this paper, a dual-signal ratiometric electrochemiluminescence (ECL) assay was developed to detect human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity. Mercaptopropionic acid (MPA)-modified Eu3+-doped CdS nanocrystals (MPA-CdSEu NCs) and luminol were used as two different ECL emitters, which showed ECL signals at different electrochemical potentials. The resultant ECL peaks exhibited opposite trends in the DNMT1 system. According to the ratio of the ECL responses of CdS to luminol, the DNMT1 activity was detected in the range of 1.0-30.0 U mL-1 in buffer solution. The limit of detection (LOD) was estimated to be 0.07 U mL-1 at a signal-to-noise ratio of 3. The assay was extended to detect the DNMT1 activity from crude lysates of cancer cells, along with the effect of DNMT1 inhibitors such as 5-aza and 5-aza-2'-dC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas
/
Técnicas Eletroquímicas
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DNA (Citosina-5-)-Metiltransferase 1
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Medições Luminescentes
Limite:
Humans
Idioma:
En
Revista:
Analyst
Ano de publicação:
2018
Tipo de documento:
Article