Overproduction of the EcoR V endonuclease and methylase.
Nucleic Acids Res
; 13(11): 3823-39, 1985 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-2989776
ABSTRACT
Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase. A 30 fold overproduction of endonuclease was achieved by randomly positioning the EndRV gene downstream of the lambda PL promoter. The situation in the endonuclease overproducing clone resembles that encountered in maxi-cells. The strains described here allowed a quick purification of both enzymes in sufficient amounts for crystallisation attempts.
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Base de dados:
MEDLINE
Assunto principal:
Desoxirribonucleases de Sítio Específico do Tipo II
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Enzimas de Restrição do DNA
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Escherichia coli
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Genes
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Genes Bacterianos
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Metiltransferases
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1985
Tipo de documento:
Article