Intramolecular H-bonds govern the recognition of a flexible peptide by an antibody.
J Biochem
; 164(1): 65-76, 2018 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-29924367
ABSTRACT
Molecular recognition is a fundamental event at the core of essentially every biological process. In particular, intermolecular H-bonds have been recognized as key stabilizing forces in antibody-antigen interactions resulting in exquisite specificity and high affinity. Although equally abundant, the role of intramolecular H-bonds is far less clear and not universally acknowledged. Herein, we have carried out a molecular-level study to dissect the contribution of intramolecular H-bonds in a flexible peptide for the recognition by an antibody. We show that intramolecular H-bonds may have a profound, multifaceted and favorable effect on the binding affinity by up to 2 kcal mol-1 of free energy. Collectively, our results suggest that antibodies are fine tuned to recognize transiently stabilized structures of flexible peptides in solution, for which intramolecular H-bonds play a key role.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Anticorpos
Idioma:
En
Revista:
J Biochem
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Japão