Study of dynamic of chicken infectious anaemia virus infection: which sample is more reliable for viral detection?
Avian Pathol
; 47(5): 489-496, 2018 Oct.
Article
em En
| MEDLINE
| ID: mdl-29974790
ABSTRACT
Chicken infectious anaemia virus (CIAV) is a widely distributed immunosuppressive agent. SPF flocks and eggs used for vaccine production and diagnostics must be CIAV-free. Detection of CIAV infection in SPF flocks involves primarily serology or other invasive methods. In order to evaluate different types of samples for rapid detection of CIAV infection, a trial was conducted in serologically negative broiler breeder pullets vaccinated with a commercial live-attenuated CIAV vaccine. Controls and vaccinated groups were sampled before and after vaccination. Invasive and non-invasive samples were used for CIAV DNA detection by real-time PCR. Seroconversion occurred at 14 days post-inoculation (DPI) in the vaccinated group, whereas CIAV genome was detected by qPCR at 7 DPI in both invasive and non-invasive samples. Only invasive samples remained qPCR positive for CIAV DNA by 21 DPI despite seroconversion of the chickens.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Doenças das Aves Domésticas
/
Galinhas
/
Infecções por Circoviridae
/
Vírus da Anemia da Galinha
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Avian Pathol
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Argentina