Changes in Synovial Fluid Biomarkers after Experimental Equine Osteoarthritis.
J Vet Res
; 61(4): 503-508, 2017 Dec.
Article
em En
| MEDLINE
| ID: mdl-29978116
ABSTRACT
INTRODUCTION:
The study aimed to clarify the changes in the concentration of inflammatory mediators, proteases, and cartilage degradation biomarkers in the synovial fluid of joints in an equine osteoarthritis model. MATERIAL ANDMETHODS:
Osteoarthritis was induced in eight Mongolian horses by a sterile intra-articular injection of amphotericin B, which was injected into the left carpal joint in a dose of 2 mL (25 mg/mL). The control group comprised five horses which were injected with an equal dose of sterile physiological saline into the left carpal joint. Synovial fluid was obtained at baseline and every week after injection. Test methods were based on ELISA.RESULTS:
In the course of the osteoarthritis, the concentration of biomarkers in joint synovial fluid showed an increasing trend. IL-1, IL-6, MMP-9, MMP-13, ADAMTS-5, CS846, GAG, HA, CTX-II, and COMP concentrations sharply increased before the onset of significant symptoms of lameness, whereas TNF-α, MMP-2, and MMP-3 concentrations rose sharply after the occurrence of such symptoms.CONCLUSION:
The results obtained confirm that the concentrations of IL-1, IL-6, MMP-9, MMP-13, ADAMTS-5, CS846, GAG, HA, CTX-II and COMP increase substantially in equine osteoarthritis, which provides a theoretical basis for the rapid diagnosis of the disease.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
J Vet Res
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
China