[The effects of macrophages with high expression of TL1A on activation and proliferation of hepatic stellate cells in vitro].
Zhonghua Gan Zang Bing Za Zhi
; 26(5): 347-352, 2018 May 20.
Article
em Zh
| MEDLINE
| ID: mdl-29996202
ABSTRACT
Objective:
To explore the effects of macrophages with high expression of TL1A on the activation and proliferation of HSCs in vitro.Methods:
The Bone marrow-derived macrophages (BMMs) and peritoneal macrophages (PMs) from wild type (WT) and myeloid-overexpressed TL1A transgenic mice were isolated, differentiated and activated. HSCs were harvested from activated macrophages culture supernatant (CM). HSCs were detected by immunofluorescence and real-time Q-PCR. And the proliferation was detected by CCK-8 and BrdU assay kit. The levels of IL-1ß and PDGF-BB in macrophage culture supernatants were determined by enzyme-linked immunosorbent assay (ELISA).Results:
BMMs-derived CM-intervention HSCs were used to detect the expression of α-smooth muscle actin (α-SMA) on the 2nd, 4th and 6th day respectively by immunofluorescence method. There was no significant difference between the two groups on the 2 nd and the 6th day, P > 0.05; On day 4, the CM/Tg group was significantly higher than that of CM/WT group, P < 0.01; the results of CMs derived from PMs were consistent with the above trend. The expression of α-SMA mRNA on the 2nd, 4th and 6th day was detected by real-time Q-PCR method using BM-derived CMs. No significant difference was found between the groups on the 2nd day (P > 0.05).α-SMA mRNA increased further on the 4th and 6th day, and the level of CM/Tg in CM/Tg group was significantly higher than that in CM/WT group (P < 0.05). The detection results of CMs derived from PMs were consistent with the above trend. The results of CCK-8 assay and BrdU assay showed that the proliferation rate of HSCs in CM Tg group was significantly higher than that in CM/WT group (P < 0.01). The CMs derived from PMs were used to interfere with HSCs. And the results were consistent with the above trend. For BMMs, the levels of IL-1ß and PDGF-BB in the lipopolysaccharide (LPS) + IFNγ/Tg culture supernatant were significantly higher than those in the LPS+IFNγ/WT group (P < 0.01). For the culture supernatants of PMs Liquid test results consistent with the above trend.Conclusion:
Macrophages with high expression of TL1A could enhance the activation and proliferation of HSCs by increasing the secretion of IL-1ß and PDGF-BB.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proliferação de Células
/
Células Estreladas do Fígado
/
Macrófagos
Limite:
Animals
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Assunto da revista:
GASTROENTEROLOGIA
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
China