Ultrasensitive label-free electrochemical immunosensor based on PVA-co-PE nanofibrous membrane for the detection of chloramphenicol residues in milk.
Biosens Bioelectron
; 117: 838-844, 2018 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-30096738
ABSTRACT
An ultrasensitive label-free amperometric immunosensor for the detection of chloramphenicol (CAP) residues in milk has been developed by using a screen-printed carbon electrode laminated with a layer of poly (vinyl alcohol-co-ethylene) (PVA-co-PE) nanofibrous membrane that is covalently immobilized with a CAP antibody (anti-CAP). The performance of the PVA-co-PE nanofiber membrane (PVA-co-PE NFM) on the electrode was compared with a PVA-co-PE casted membrane (PVA-co-PE CM), necessary fabrication steps and performance of the sensors were investigated by electrochemical impedance spectroscopy (EIS). The application of the PVA-co-PE NFM decreased the electron-transfer-resistance by about 4 times compared with a conventional PVA-co-PE casted membrane. Under the optimal conditions, the established immunosensor exhibited high sensitivity for determination of CAP in a range 0.01-10â¯ngâ¯mL-1, with a limit of detection of 0.0047â¯ngâ¯mL-1. In addition to the good selectivity, reusability and stability over time, the prepared immunosensor was successfully used in the detection of CAP in milk samples without any pretreatment.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Álcool de Polivinil
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Imunoensaio
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Resíduos de Drogas
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Cloranfenicol
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Polietileno
/
Leite
/
Técnicas Eletroquímicas
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Nanofibras
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biosens Bioelectron
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article