Detection of Necroptosis by Phospho-RIPK3 Immunohistochemical Labeling.
Methods Mol Biol
; 1857: 153-160, 2018.
Article
em En
| MEDLINE
| ID: mdl-30136239
Activation of the kinase RIPK3 (receptor interacting protein kinase 3) is a hallmark of cells dying by necroptosis. RIPK3 phosphorylates both itself and the pseudokinase MLKL (mixed lineage kinase-like) resulting in MLKL translocation to membranes and cell lysis. Antibodies recognizing RIPK3 autophosphorylation or the RIPK3-dependent phosphorylation sites on MLKL have therefore been used to monitor necroptosis induction. Here we describe immunohistochemical labeling for autophosphorylated mouse RIPK3 as a means of detecting cells undergoing necroptosis in mouse tissues.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Imuno-Histoquímica
/
Apoptose
/
Embrião de Mamíferos
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Proteína Serina-Treonina Quinases de Interação com Receptores
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Necrose
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Estados Unidos