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A metabolic and genomic assessment of sugar fermentation profiles of the thermophilic Thermotogales, Fervidobacterium pennivorans.
Wushke, Scott; Fristensky, Brian; Zhang, Xiang Li; Spicer, Vic; Krokhin, Oleg V; Levin, David B; Stott, Matthew B; Sparling, Richard.
Afiliação
  • Wushke S; Department of Microbiology, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
  • Fristensky B; Department Plant Science, University of Manitoba, Winnipeg, MB, Canada.
  • Zhang XL; Department Plant Science, University of Manitoba, Winnipeg, MB, Canada.
  • Spicer V; Department Physics and Astronomy, University of Manitoba, Winnipeg, MB, Canada.
  • Krokhin OV; Department of Internal Medicine and Manitoba Centre for Proteomics and Systems Biology, University of Manitoba, Winnipeg, MB, Canada.
  • Levin DB; Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB, Canada.
  • Stott MB; Geomicrobiology Research Group, GNS Science, Wairakei, New Zealand.
  • Sparling R; School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
Extremophiles ; 22(6): 965-974, 2018 Nov.
Article em En | MEDLINE | ID: mdl-30182148
A metabolic, genomic and proteomic assessment of Fervidobacterium pennivorans strains was undertaken to clarify the metabolic and genetic capabilities of this Thermotogales species. The type strain Ven5 originally isolated from a hot mud spa in Italy, and a newly isolated strain (DYC) from a hot spring at Ngatamariki, New Zealand, were compared for metabolic and genomic differences. The fermentation profiles of both strains on cellobiose generated similar major end products (acetate, alanine, glutamate, H2, and CO2). The vast majority of end products produced were redox neutral, and carbon balances were in the range of 95-115%. Each strain showed distinct fermentation profiles on sugar substrates. The genome of strain DYC was sequenced and shown to have high sequence similarity and synteny with F. pennivorans Ven5 genome, suggesting they are the same species. The unique genome regions in Ven5, corresponded to genes involved in the Entner-Doudoroff pathway confirming our observation of DYC's inability to utilize gluconate. Genome analysis was able to elucidate pathways involved in production of the observed end-products with the exception of alanine and glutamate synthesis which were resolved with less clarity due to poor sequence identity and missing critical enzymes within the pathway, respectively.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos / Genoma Bacteriano / Açúcares / Fermentação Idioma: En Revista: Extremophiles Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Canadá

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bacilos Gram-Negativos Anaeróbios Retos, Helicoidais e Curvos / Genoma Bacteriano / Açúcares / Fermentação Idioma: En Revista: Extremophiles Assunto da revista: BIOLOGIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Canadá