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Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer.
Cao, Liwei; Pauthner, Matthias; Andrabi, Raiees; Rantalainen, Kimmo; Berndsen, Zachary; Diedrich, Jolene K; Menis, Sergey; Sok, Devin; Bastidas, Raiza; Park, Sung-Kyu Robin; Delahunty, Claire M; He, Lin; Guenaga, Javier; Wyatt, Richard T; Schief, William R; Ward, Andrew B; Yates, John R; Burton, Dennis R; Paulson, James C.
Afiliação
  • Cao L; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Pauthner M; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Andrabi R; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Rantalainen K; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Berndsen Z; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Diedrich JK; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Menis S; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Sok D; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Bastidas R; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Park SR; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Delahunty CM; Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • He L; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Guenaga J; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Wyatt RT; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Schief WR; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Ward AB; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Yates JR; Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Burton DR; Center for HIV/AIDS Vaccine Immunology and Immunogen Discovery and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA, 92037, USA.
  • Paulson JC; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, 92037, USA.
Nat Commun ; 9(1): 3693, 2018 09 12.
Article em En | MEDLINE | ID: mdl-30209313
ABSTRACT
As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans. Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design. Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers. However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity. These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / HIV-1 Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polissacarídeos / HIV-1 Limite: Humans Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Estados Unidos